Journal: Journal of Neuroinflammation
Article Title: Intrauterine inflammation and postnatal intravenous dopamine alter the neurovascular unit in preterm newborn lambs
doi: 10.1186/s12974-024-03137-0
Figure Lengend Snippet: Desmin and αSMA positive immunohistochemistry ( A ) Quantification of percentage of colocalization of desmin with αSMA positive immunolabelling. Data are mean ± SD. Two-way ANOVA. ( E ) Representative images of desmin (green) and αSMA (red) positive immunofluorescent labellingand colocalization indicating pericyte coverage of vessels in the cortical grey periventricular white matter (PVWM). Scale bar represents 50 μm
Article Snippet: To identify pericytes, sections were incubated in mouse monoclonal α-smooth muscle actin (α-SMA, 1:50; Sigma, USA; CAT#:A5228) and rabbit polyclonal anti-desmin (1:50; Sigma, USA; CAT#:D8281).
Techniques: Immunohistochemistry